علوم زیستی دریا
Marjan Naseri Karimvand; yadollah nikpour; Ahmad Taghavi Moghadam; Kamal Ghanemi
Abstract
Gelatin is polypeptide with high molecular weight which is derived from collagen of connective tissue, skin, bone and tendons. The most common source of gelatin in the world is skin and bone of cow and pig. Gelatin from persian Gulfs jellyfish Crambionella orsini have been successfully extracted by alkaline ...
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Gelatin is polypeptide with high molecular weight which is derived from collagen of connective tissue, skin, bone and tendons. The most common source of gelatin in the world is skin and bone of cow and pig. Gelatin from persian Gulfs jellyfish Crambionella orsini have been successfully extracted by alkaline extraction.We investigated the proximate composition, gel strength, gelling point, melting point of jellyfish gelatin. Jellyfish gelatin contained 13.1% moisture, 1.3% lipid, 2.4% ash, 78.2% protein. The gelatin showed a gel strength of 33 kPa, a gelling point of 18°C, and melting point of 23°C. The gelatin was composed of α1-chain, α2-chain, β-chain, and γ-chain Jellyfish gelatin did not show superior rheological properties to mammalian gelatin, like other fish gelatin; however, it can be used in various food and cosmetic products not requiring high gel strength.
علوم زیستی دریا
M. Parviz; yadollah nikpour; A. Taghavi Moghadam; K. Ghanemi
Abstract
In this paper, the nematocyst venom of jellyfish crambionella orsini were fractionated by size-exclusion and anion-exchange chromatography. crambionella orsini is famous jellyfish in Persian gulf. The results of mentioned methods has been investigated. The crambionella orsini venoms has hemolytic activity, ...
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In this paper, the nematocyst venom of jellyfish crambionella orsini were fractionated by size-exclusion and anion-exchange chromatography. crambionella orsini is famous jellyfish in Persian gulf. The results of mentioned methods has been investigated. The crambionella orsini venoms has hemolytic activity, as the other species.After extraction of nematocyst venom, the crude venom has been partially purification by sephadex G-200 gel filtration and DEAE anion exchange chromatography. Protein elution was monitored by uv detection at 280 nm. For determine the hemolytic fraction, every fraction were injected to 3 mice via the tail vein. Finally, all data from both chromatography methods were compared. The gel filtrations first pick and the first and second pick of anion exchange chromatography showed hemolytic activity.Determination the appropriate method for purification of this venom can help to find a comprehensive method for another marine venom especially jellyfish venoms and eventually may be help to find specific antidote for jellyfish stings of these species.